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對各種生物材料的超聲波照射

時間:2018-1-23 23:03:58作者:admin1

對各種生物材料的超聲波照射

Name	Description
Actinomyces	3 minutes of sonifying produces excellent disruption with 50% protein release and excellent enzyme activity.
Actinomycin D	Suspended in 3 minutes.
Aerobacter Aerogenes	Excellent breakage with better enzyme release than any other method. A low power setting can release sulfatase activity into the supernate with no obvious disruption of the majority of cells.
Aerobacter Suboxydans	Excellent breakage but requires higher power than aerogenes.
Algae Secendesmus	10 ml concentrated solution completely disrupts in 1 minute.
Alkaloids	Total amount and speed of extraction are greater than with standard methods. Extraction from ipecac root in 30 seconds yielded more alkaloid than Soxhlet extraction in 5 hours.
Antibioticus	Monocellular elements from surface-grown colonies obtained in 1 minute. Complete disruption in 5 minutes, 50% disruption in 2 minutes.
Antigen	The Sonifier system is used extensively to produce antigens and vaccines. It can increase yield or expose otherwise unobtainable sites.
Aorta	1 gram disintegrates in 2 minutes.
Aphanomyces	After blending, complete disruption in 3 minutes.
Arthobacter Tumescens	10 gm in 40 ml in 5 minutes for O coumaric reductose.
Ascaris Eggs	8 ml concentrated solution completely disrupts in 4 minutes.
Asperigillus	Complete disruption in 4 minutes.
Aurefaciens	Monocellular elements from surface-grown colonies obtained in 1 minute. Complete disruption in 5 minutes, 50% disruption in 2 minutes.
Azotobacter Vinelandii	15 ml buffered solution, 200 mg wet wt/ml completely disintegrates in 2 minutes.
B. Anthracis	80% disruption of anthracis in 4 minutes. Complete disruption of 10 ml of eryisipelothrix rhusipathiae in 10 minutes.
B. Cereus Veg Cells	Disruption in a few seconds.
B. Cereus Spores	Disruption of 10 mg/6 ml in 13 minutes.
B. Megaterium Spores	Complete breakage of a concentrated 6 ml solution in 15 minutes.
B. Sphaericus	Major disruption in 1-3 minutes.
B. Stereothermophilis Spores	Complete disruption in 2 minutes.
B. Subtilis	Disruption of 5 gm wet wt, 15 ml buffer, in 5 minutes.
B. Subtilis Veg Cells	Heavy suspension clears in 1 minute.
Bacillus Stereothermophulus (Thermophillic Spore Form)	98% disruption of 70 ml of 40% suspension in 15 minutes.
Bacillus Brevis	1:15 W/V in 3 minutes.
Bacteroides Symbiosis	1-phosphorfructokinase (a soluble enzyme) has been isolated from this anaerobe by ultrasonic treatment. A 25 ml suspension was sonified for 10 minutes and centrifuged at 36,000 xg for 10 minutes.
Baker’s Yeast (Saccharomyces Cerevisiae)	See Yeast.
Blastomyces Dermatitidis	95% disruption in 3 minutes.
Blood Cells	Red and white cells can be disrupted in a few seconds.
Boll Weevil Tissue	Complete homogenization in a few seconds.
Bone	Compact bone can be sonified and processed for microscopic sections in minutes. Other methods can require up to a week. Bone specimens treated in this way yielded large numbers of intact cells with little distortion. Malignant criteria are easily recognized. Tumor types studied were: osteosarcoma, chondrosarcoma, liposarcoma, chordoma, metastic bronchogenic squamous and benign giant. Bone can be decalcified without injury to the cells, processed for microscopic sections, and diagnosed in a short time. Other methods require extensive treatment time.
Brain Stem And Adrenal Gland	Ultrasonic treatment dispersed 10 mg samples in 10 ml fluid, which is usually difficult without substantial loss of material. The suspension was analyzed for nucleotides.
Brain Tissue	Disintegrates instantly.
Brevi Bacterium	25 ml disrupts in 20 seconds.
Brevi Bacterium Acetylicum	Approximately 3 minutes to disrupt large samples and measure TCA enzyme activity.
Brine Shrimp	Complete disintegration in 1 minute.
Brucella Abortes	Separates easily from leucocytes. At least 9 antigens extracted.
Bull Sperm	Contractile protein is easier to extract from tails after sonifying.
C. Butyricum, C. Cylinrosporum, C. Kluyveri	Vegetative cells easily disrupted.
C. Pasteurianum	3 minutes disruption for hydrogens reducing Ferredoxin with H2.
Calcium	Mouse Ehrlich ascites tumor cells were sonified for 1 minute to determine the amount of bound calcium present. Cells were labeled with calcium 45.
Candida Albicans Spores	95% disruption of 1/2 gram dry wt. in a 15 ml solution in 35 minutes.
Carbon Black	Excellent small particle suspension.
Caryophanon Latum	Glucosamine, muramic acid, alanine, glutamic acid and lysine were obtained.
Catecholamine	Can be extracted from heart muscle.
Cellumonas Biazotea	Disruption obtained with retention of malate dehydrogenase activity. Chicken spermatozoa: completely disrupts in 2 minutes.
Chlorella	Completely disrupts in 3 minutes.
Chloroplasts	Disrupt in a few seconds.
Cholesterol	Apparent permanent suspension in 1 minute in water.
Desullovibrio Vulgaris	Less than 30 seconds of ultrasonic treatment resulted in release of TCA enzymes.
Diplococcus	Completely disrupts in 5 minutes.
Dna	Breaks chains on low power instantly. Controlled degradation may be obtained.
Dyes	Excellent rapid dispersion and homogenization.
E. Coli.	2 gm wet weight in 10 ml solution completely disrupts in 40 seconds. The Sonifer Cell Disruptor has been used extensively in research on this organism.
Egg Whites	Can be reduced to a homogeneous pipettable solution in 15 seconds on low power.
Ehrlich Ascites	Disrupts in a few seconds.
Electron Microscopy	Apertures are quickly cleaned.
Emulsions	10 ml of most light mixtures become semi-permanent emulsions in about 1 minute without emulsifiers. Average particle size is usually well under 1 micron. Sterile emulsions can be prepared by ultrasonic treatment for feeding to germ-free animals.
Enterococcus	Excellent disruption.
Erwina Cartovara	Complete disruption in 1-2 minutes depending on cell concentration.
Erythrocytes	Disrupts in a few seconds.
Euglena Gracilis	Completely disrupts in a few seconds to isolate chloroplasts.
Eugoena	Complete disruption in 12 minutes, 90% disruption in 8 minutes with pigment released.
Fat Extraction	Fat can be emulsified without injuring tissue with proper power selection. Lipid layer can be stripped from spores and mycobacteria.
Fibrin	Complete suspension of 0.125 gm in 30 minutes.
Fish Gill	Complete disruption of 20 mg in 30 seconds.
Fish Tissue	Tissue homogenization for extractions and excellent particle size reduction in 8 minutes per 10 gm.
Fluorocarbons	Extended treatment time will break down particle size to well under 1 micron and gives a fine homogenate.
Fossils	Low power will clean debris from delicate fossils without injury. Micro fossils such as pollen can be separated from rocks to help identify the geological age of the strata. Removal of rock matrix.
Gamma Globulin	The Sonifer Cell Disruptor was used to solubilize protein as one of the steps in the biosynthesis of gamma globulin from rabbit spleen.
Gangliosides	Immunochemical and structure studies used ultrasonic treatment as one step.
Gastric Mucosa	Placing scrapings into a test tube and test tube into new water- filled cup horn caps permits these cells to be separated without breakage.
Graphite Molybdenum Disulfide	An excellent dispersion of this lubricant was made in a silicate binder.
Guanine	Produces colloidal suspension in 1 minute.
Gymnodinium	Solution completely disrupts in 6 minutes.
Haemophilus Pertussis	Preparation of successful immunological complexes.
Heart Muscle	1 gm disintegrates in 6 minutes.
Hela Cells	Disruption to free virus in a few seconds without injury.
Hemophilus Pertussis	An immunological compound prepared.
Herpes Virus	May be quickly released without injury.
Histoplasma Capsulatum	Ultrasonic treatment for 7 minutes completely ruptures cells prepared by formalin fixation. Good enzyme activity is obtained.
Human Serum Proteins	Ultrasonic treatment causes a reproducible change in the electrophoretic behavior of normal human serum consisting of an increase in material migrating in the x and b globulin zones with a reduction in the albumin and y globulin fractions.
Hydrocortisone	Smaller crystals were produced by ultrasonic treatment.
Hydrophilic Vegetable Gums	Disperses and solubilizes hydrophilic vegetable gums in water;makes dispersions of added particulate matter.
Intracellular Membrane	Disruption and particle size reduction obtained in 30-60 seconds.
Isoenzymes	Selectively activated with respect to time and intensity of treatment.
Kidney	1 gm disintegrates in 3 minutes.
Kidney Stones	Easily broken in seconds in vitro.
Klebsiella	Excellent disruption.
L. Arabinosis	Complete disruption to free virus in 2 minutes without injury.
Lactobacillus	0.5 gm in 15 ml completely disrupts in 11 minutes. Excellent release of acetokinase.
Lenconostoc Mesenteroides	Ultrasonic treatment for 15 minutes using high power for disruption.
Leukocyte Lysozyme Activity In Myelocytic Leukemia	The cell suspension was ultrasonically treated and samples assayed for lysozyme activity. The lysozyme concentration of the leukocytes ug/106 cells was determined.
Linoleic Acid	Made suspension in water in 30 seconds.
Liver Tissue	1 gm homogenizes in less than 1 minute.
Lung Tissue	1 gm homogenizes in 2 minutes.
Lymphacytis	Complete disruption in 15 seconds.
Lymphocyte Nuclei	Complete disruption in 6 minutes.
Lymph Gland	Cirect injection lymphography with a modified radiopaque emulsion was obtained by ultrasonic treatment in a functional procedure producing lymphatic structure detail.
Lysossomes	Released enzymes quickly.
Malaria Prolozoa	Fast, excellent disruption.
Maple Bark Spores	Complete disruption in 14 minutes.
Measles	Disruption of virus antigen clumps present in infected cells on low power. Ultrasonic treatment increased antigen titer 4-8 fold.
Methanobacillus Omelianskii	1 gm cells wet wt/ml completely disintegrates in 2 minutes for assaying methane.
Microbacterium Lacticum	Ultrasonic treatment used for malate dehydrogenase extraction.
Micrococci	A 13 ml solution completely disrupts in 15 minutes.
Micrococcus Lactiliticus	75 ml of a 20% suspension was disintegrated in 15 minutes and a good yield of the enzyme Xanthine dehydrogenase extracted.
Mineral Rock	Excellent for cleaning surfaces between polishing stages.
Mitochondria	Separate from cells without injury. Mitochondria themselves can be broken with longer ultrasonic treatment. Inner membrane subunits also isolated.
Muscle Tissue	1 gm homogenized in 4 minutes; heart muscle in 6 minutes.
Mycobacteria	A 20 ml growing media completely disrupts in 14 minutes. Clumps break quickly. An immunological compound prepared.
Mycoplasma Antibody	A suspension of Campo-W cells treated for 5 minutes gave 12 lines with the sera in a gel diffusion test. The extract was estimated to contain 12.75 mg protein per ml by Blaret reaction.
Myeloma Tumor Cells	Complete disruption in 10 minutes, 30% disruption in 2 minutes.
Myleran	Made colloidal suspension and dissolved in approximately 1 minute.
N. Crassa	Nuclease was isolated and purified from conidial extracts after 5 minutes treatment.
Naegleri Gruberi	This free-living soil amoeba was treated ultrasonically to release subcellular infectious material.
Neurospora	40 ml, 4 minutes, produced more protein than freeze thawing for study of enzymatic synthesis of cystathionine.
Nocardia Ostenodes	Breaks clumps and disintegrates in less than 10 minutes.
Nucleoprotein	Extracted from tissue. May be degraded selectively.
Oil And Water Emulsions	Permanent, stable emulsions in a few seconds. Particle size reduced to less than micron (each case slightly different). Oil in water/water in oil phases can be obtained in same vessel.
Oyster Shell	Small, clean hole can be drilled with microtip in 3 minutes. No cracking is produced.
Paracolon	Excellent disruption.
Parasites	Separated from red blood cells in a few seconds.
Pasteurella Pestis	Complete disruption in 30 minutes using high power.
Penicillium	Complete disruption in 3 minutes.
Pesticides	Ultrasonic treatment resulted in a 16-fold improvement in the potency of the antigen used with Microcrystalline Cellulose as a thin-layer absorbent for chromatographic separation.
Phosphatidate Phosphohydrolase	The most potent inhibitors for this enzyme were obtained by making five dispersions with the Sonifier system.
Phospholipid Micelles	Produced stable preparations for an indefinite period.
Plant Cells	30% packed plant cells (W/V) and distilled water (depending on type) can be completely disrupted in 1-15 minutes.
Plant Tissue	1 gm dried tissue suspended in alcohol disintegrates in about 5 minutes.
Platelets	Complete disruption according to size from 20 seconds to 4 minutes.
Pneumococci	Preserved in formalin for several years; completely disrupts in 6 minutes.
Polio Virus	Excellent disruption of this most difficult virus.
Powders	Broken down to a small, relatively uniform particle size.
PPLO	Complete disruption in 2 minutes.
Propionobacteria	Releases sulfhydro groups intact; 70 ml of 20% suspension processed for 10 minutes.
Propionibacterium Shermanii	2 minutes for extraction of citrate synthose.
Proteus	Excellent disruption.
Pseudomonas Aeruginosa	Rapid, complete disintegration.
Pseudomonas Fluorescens	2 gm wet wt in 10 ml completely disrupts in 1 minute.
Pulmonary Cytodiagnosis	The mucous in sputum can be evenly dispersed, giving a quick representative sample of cells for cytologic examination. Cells are liberated from the mucous of sputum that had been immersed in 50% alcohol or a fixative.
Ragweed Pollen	15 ml dispersion completely disintegrates in 11 minutes.
Rat Bone	1/2 gm disintegrates in 4 minutes.
Rat Liver	Complete disruption in 3 minutes.
Rat Liver Mitochondria	Ultrasonic treatment has been used extensively for the varied research performed on this material. Disruption occurs in seconds.
Rat Skin	1 gm completely disintegrates in 4 minutes.
Red And White Blood Cells	Ultrasonic treatment breaks particle size to 100 Angstroms. Complete disruption in 1 minute. 25 gms/100 ml, saline or plasma, sample treated 15 seconds, 35% disruption. Adenosine triphosphate was shown to be membrane-bound by this method.
Reovirus	Dissociates cell-bound and aggregated virus. Maximum titer with 4 ml of virus was achieved in 2 minutes.
Retinal Outer Segments	Ultrasonic treatment breaks particles down to almost molecular size.
Rhodopseudomonis Palustris	Complete disruption in 4 minutes.
Rhodospirillum Rubrum	Excellent disruption in a few seconds.
Rimosus	Monocellular elements from surface-grown colonies obtained in 1 minute. Complete disruption in 5 minutes, 50% disruption in 2 minutes.
RNA	Rapid and thorough re-suspension of 9 TCA pellets during extractions.
Rocks	Excellent for disaggregation of sedimentary rock and for cleaning material rock surfaces between polishing stages.
S. Faecalis	Excellent disruption in 1 minute.
S. Fragilis	5 minutes yielded excellent release of galactokinase, more than any other method. Subcellular particles may be extracted or disrupted.
Saliva Glands	Complete disruption.
Salmonella	Various culture media or phosphate buffered saline disintegrated between 40 and 50% in 10-20 minutes. Sonifying was one step in an improved assay for enzyme thiogalactosize transacetylase.
Salmonella Typhimurium And Enteritidis	Bacteria were suspended in 1/300 volume of original culture, sonified for 4 minutes and centrifuged for 20 minutes at 20,000 g. Extracts were found to catalyze the synthesis of cytidine diphosphate 3, 6-dideozyhexoses.
Scholasticism Mansion	Complete disruption.
Sedimentary Rock	Completely disperses flocs with the release of all bound silt and clay particles.
Sediments	Ultrasonic treatment disperses fine material permitting quick, neat separation of sand from silt and clay fractions.
Serial Number Restoration	Used in crime laboratories to restore obliterated serial numbers.
Serratia Marcescens	Complete breakdown of a concentrated solution in 1 minute.
Serum	Quickly homogenized.
Serum Cholinesterase	Activated by ultrasonic treatment. Different cholinesterase isoenzymes may be activated and inactivated selectively.
Shale	Excellent disaggregation of all fine-grained sedimentary rocks.
Shellfish	By drilling a clean hole with the microtip, various fluids or samples may be withdrawn or injected from living shellfish without destroying the animals.
Shigella	Quick disruption.
Skin	1 gm disintegrates in about 4 minutes. Epidermal homogenates that respire and utilize substrate can be extracted.
Soil	Separates solid particles without use of oxidants, acids or peptizing agents and yields stable suspensions.
Sperm (Human)	Tails are broken instantly. Heads are broken in 20 minutes.
Sputum	Cancer cells are more easily detected after ultrasonic treatment due to even dispersion of cells and sputum, and complete liberation of the cells from sputum.
Staphylococcus	A concentrated solution disrupts 98% in 10 minutes. With 1 gm cells wet wt, to 2 gm water, 54.5 mg/ml of protein was released.
Starch	Obtained by extracting from green plant leaf homogenate.
Streptococcus, Group A	A 20% suspension in a 15 ml solution completely disrupts in 15 minutes.
Streptomyces	Monocellular elements from surface-grown colonies obtained in 1 minute. Complete disruption in 5 minutes, 50% disruption in 2 minutes.
Subcellular Particles	May be separated or broken depending on power selection and length of time.
Sulfanilamide	Excellent dispersion in less than 1 minute. Continued ultrasonic treatment will produce complete disruption.
Sulfapyridine	Excellent dispersion in less than 1 minute. Continued ultrasonic treatment will produce complete disruption.
Synovial Fluid	Ultrasonic treatment is an excellent means of reducing fluid viscosity. It is simpler and faster than using hyaluronidase.
T. Pyriformi	Excellent disruption, 8 enzymes released.
Tablets	Complete disruption in 2-40 seconds, depending on type.
Tea	Excellent extraction.
Tetrahymena	Disrupts in a few seconds. Enzymes which have been monitored include: succinate, lactate, B-hydroxy butyrate, glutamate and DPNH oxidases, DPNH-cytochromeC, reductase and ribonuclease. Activity of DPNH oxidase was twice that of the best previous experiments.
Themoactinomyces	Disruption of hyphae. Homogenization of protein complex without denaturation.
Thermophile Negative	Good disruption within 2 minutes.
Thermophilic Bacillus	Isocitrate lyase was extracted from a spore-forming bacillus similar to Stearothermophilus. A washed cell paste suspended in a phosphate buffer was sonified 1-2 minutes and the supernatent was used for enzyme experiments without further treatment. Extracts could be frozen and stored without loss of activity.
Thiouric Acid	Dissolved in a few seconds.
Thymus Cells	Complete disruption in 15 seconds.
Tissue Culture Cells	Complete disruption in a few seconds. To avoid damage to free organelles and to obtain intact lysosomes, use low power at short exposure.
Toxin And Antitoxin	One example of many: Toxin preparations of whole cell lysate (WCL) of the Inaba serotype strain 569E of the classic biotype of cholera vibrio were grown on 3% Bacto peptane agar and harvested in distilled water at 18 hours. The unwashed suspensions were solubilized ultrasonically, clarified by centrifugation and the supernate freeze-dried for the titration of cholare toxin in the rabbit ileal loop.
Toxoplasma Gondii	Can be separated form white blood cells without injury.
Transplantation Antigens	Were extracted from spleen, thymus and lymph nodes.
Trichomonas Foetus	Complete disruption in a few seconds.
Triolein	Complete stable emulsion in 2 minutes.
Trypanosomes	Concentrated 10 ml solution completely disrupts in 4 minutes.
Uterus Muscle	A 1/5 gm, 3 cc solution completely disrupts in 3 minutes for coenzyme Q determination.
Vaccines	Numerous advantages, such as more antigenic material released than usual, and the production of vaccines not obtainable by classified methods.
Various Bacilli	Complete disruption in 3 minutes.
Vibrio Comma	Excellent disruption.
Vibrio Extraction	Excellent for experimental vaccines. Evidence of breakage of virus/antibody bonds. Virus can be extracted without damage at low power, or broken at high power.
Vitamin E	30 seconds of ultrasonic treatment put material in solution with a resultant permanent suspension.
W138 Virus	Cell-free V-2 virus obtained in 30 seconds using 6 ml of Veronal buffer with W138 cells containing V-2 virus.
Yeast	Complete disruption in 3-10 minutes. Complete disruption of 9 grams pressed yeast in 18 ml buffer in 8 minutes. Protein release,52 mg/ml from an aged sample.

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